RabbitPolyclonalAntibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Bovine,Canine,Chicken,Human,Mouse,Non-humanprimate,Rat,Sheep,Xenopus,Zebrafish
- Applications:
- WB1:1000IHC1:500
- Species:
- Rabbit
- MolecularReference:
- ~29kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1433-58,RRID:AB_2492027
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer58ofrat14-3-3protein.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
14-3-3proteinsareafamilyofhighlyconservedproteinsthatappeartohavemultiplerolesincellsignaling(BridgesandMoorhead,2005).Theproteinsareabundantlyexpressedinthebrainandhavebeendetectedinthecerebrospinalfluidofpatientswithdifferentneurologicaldisorders(Bergetal.,2003).14-3-3proteinsbindproteinligandsthataretypicallyphosphorylatedonserineorthreonineresiduesandregulatethefunctionsofthesebindingpartnersbyanumberofdifferentmechanisms(Silhanetal.,2004;DoughertyandMorrison,2004).The14-3-3proteinsaffectadiversearrayofcellularprocessesincludingthecellcycleandtranscription,signaltransductionandintracellulartrafficking.Thesefunctionsof14-3-3proteinsarefacilitatedby,ifnotdependenton,itsdimericstructure.Recentworkhasdemonstratedthatthedimericstatusofthe14-3-3proteinisregulatedbysite-specificserinephosphorylation(Woodcocketal.,2003)
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
XiangjunYang,ChengLuo,JianCai,WilliamM.Pierce,andGülgünTezel(2008)Phosphorylation-DependentInteractionwith14-3-3intheRegulationofBadTraffickinginRetinalGanglionCells.Invest.Ophthalmol.Vis.Sci.,49:2483-2494. PMID:18296656
