RabbitPolyclonalAntibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1000IHCNotrecommendedforuseinparaffinsections
- Species:
- Rabbit
- MolecularReference:
- ~29kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1433-P,RRID:AB_2492026
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheN-terminalregionoftherat14-3-3protein,conjugatedtokeyholelimpethemocyanin(KLH).
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviachromatographyonanaffinitycolumnpreparedwiththeN-terminalpeptideusedasantigen.
BIOLOGicalSignificance:Collapse
14-3-3proteinsareafamilyofhighlyconservedproteinsthatappeartohavemultiplerolesincellsignaling(BridgesandMoorhead,2005).Theproteinsareabundantlyexpressedinthebrainandhavebeendetectedinthecerebrospinalfluidofpatientswithdifferentneurologicaldisorders(Bergetal.,2003).14-3-3proteinsbindproteinligandsthataretypicallyphosphorylatedonserineorthreonineresiduesandregulatethefunctionsofthesebindingpartnersbyanumberofdifferentmechanisms(Silhanetal.,2004;DoughertyandMorrison,2004).The14-3-3proteinsaffectadiversearrayofcellularprocessesincludingthecellcycleandtranscription,signaltransductionandintracellulartrafficking.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
IrinaSurgucheva,ValeryI.Shestopalov,andAndreiSurguchov(2008)Effectofgamma-SynucleinSilencingonApoptoticPathwaysinRetinalGanglionCellsJ.Biol.Chem.,283:36377–36385.PMID:18936092
