Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1000
- Species:
- Rabbit
- GeneName:
- AQP2
- MolecularReference:
- ~29kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p112-264,RRID:AB_2492042
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser264ofrataquaporin2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Aquaporin2(AQP2)isahormonallyregulatedwaterchannellocatedintherenalcollectingduct.MutationsintheAQP2genecausehereditarynephrogenicdiabetesinsipidusinhumans(Iolasconetal.,2007).AvasopressininducedcAMPincreaseresultsinthephosphorylationofAQP2atserine-256anditstranslocationfromtheintracellularvesiclestotheapicalmembraneofprincipalcells(vanBalkometal.,2002).Recently,serine-264hasbeenidentifiedasanovelphosphorylationsiteonAQP2andshowntoberegulatedbyvasopressinthusimplicatingthissiteasimportantinAQP2traffickingandsubcellulardistribution(FentonRAetal.,2008).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Lei,L.,Huang,M.,Su,L.,Xie,D.,Mamuya,F.A.,Ham,O.,Tsuji,K.,Păunescu,T.G.,Yang,B.andLu,H.A.J.,2017.ManganesepromotesintracellularaccumulationofAQP2viamodulatingF-actinpolymerizationandreducesurinaryconcentrationinmice.AmericanJournalofPhysiology-RenalPhysiology,Oct18:ajprenal-00391.PMID:29046300
Al-Bataineh,M.M.,Li,H.,Ohmi,K.,Gong,F.,Marciszyn,A.,Naveed,S.,Zhu,X.,Neumann,D.,Wu,Q.,Cheng,L.,&Fenton,R.A.(2016).ActivationoftheMetabolicSensorAMP-ActivatedProteinKinaseInhibitsAquaporin-2FunctioninKidneyPrincipalCells.AmericanJournalofPhysiology-RenalPhysiology,ajprenal-00308. PMID:27534994
Speed,J.S.,&Hyndman,K.A.(2016).Invivoorganspecificdrugdeliverywithimplantableperistalticpumps.Scientificreports,6.PMID:27185292
Choi,H.J.,Jung,H.J.,&Kwon,T.H.(2015).ExtracellularpHaffectsphosphorylationandintracellulartraffickingofAQP2ininnermedullarycollectingductcells.AmericanJournalofPhysiology-RenalPhysiology,308(7),F737-F748.PMID:25651562
Rice,W.L.,Li,W.,Mamuya,F.,McKee,M.,Păunescu,T.G.,&Lu,H.A.J.(2015).PolarizedTraffickingofAQP2RevealedinThreeDimensionalEpithelialCulture.PloSone,10(7),e0131719.PMID:26147297
Hoffert,J.D.,Pisitkun,T.,Saeed,F.,Wilson,J.L.,&Knepper,M.A.(2014).GlobalanalysisoftheeffectsoftheV2receptorantagoNISTsatavaptanonproteinphosphorylationincollectingduct.AmericanJournalofPhysiology-RenalPhysiology,306(4),410-421.PMID: 24259510
HoffertJD,FentonRA,MoellerHB,SimonsB,TchapyjnikovD,McDillBW,YuMJ,PisitkunT,ChenF,KnepperMA.(2008)Vasopressin-stimulatedincreaseinphosphorylationatserine269potentiatesplasmamembraneretentionofaquaporin2.JBiolChem.2008Sep5;283(36):24617-27PMID:18606813
Fenton,R.A.,Moeller,H.B.,Hoffert,J.D.,Yu,M.J.,Nielsen,S.,&Knepper,M.A.(2008).Acuteregulationofaquaporin-2phosphorylationatSer-264byvasopressin.ProceedingsoftheNationalAcademyofSciences,105(8),3134-3139.PMID:18287043
HoffertJD,NielsenJ,,YuMJ,PisitikunT,SchleicherSM,NielsenS,KnepperMA(2007)Dynamicsofaquaporin-2serine-261phosphorylationinresponsetoshort-termvasopressintreatmentincollectingduct.AmJPhysiolRenalPhysiol292:F691-F700.PMID:16985212
