Rabbit polyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Bovine,Canine
- Applications:
- WB1:1000
- Species:
- Rabbit
- GeneName:
- AQP2
- MolecularReference:
- ~29kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p112-269,RRID:AB_2492043
Antigen/Purification:Collapse
Theantibodyisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser269ofrataquaporin2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Aquaporin2(AQP2)isahormonallyregulatedwaterchannellocatedintherenalcollectingduct.MutationsintheAQP2genecausehereditarynephrogenicdiabetesinsipidusinhumans(Iolasconetal.,2007).AvasopressininducedcAMPincreaseresultsinthephosphorylationofAQP2atserine-256anditstranslocationfromtheintracellularvesiclestotheapicalmembraneofprincipalcells(vanBalkometal.,2002).Serine-269hasbeenrecentlyidentifiedasavasopressin-mediatedphosphorylationsiteonAQP2andassuchhasshowntopotentiateplasmamembraneretentionofAQP2(HoffertJDetal.,2008).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Lei,L.,Huang,M.,Su,L.,Xie,D.,Mamuya,F.A.,Ham,O.,Tsuji,K.,Păunescu,T.G.,Yang,B.andLu,H.A.J.,2017.ManganesepromotesintracellularaccumulationofAQP2viamodulatingF-actinpolymerizationandreducesurinaryconcentrationinmice.AmericanJournalofPhysiology-RenalPhysiology,Oct18:ajprenal-00391.PMID:29046300
Yui,N.,Ando,F.,Sasaki,S.andUchida,S.,2017.Ser-261phospho-regulationisinvolvedinpS256andpS269-mediatedaquaporin-2apicaltranslocation.BiochemicalandBiophysicalResearchCommunications.2017Jun28. PMID:28668390
Yui,N.,Sasaki,S.andUchida,S.,2016.Aquaporin-2Ser-261phosphorylationisregulatedincombinationwithSer-256andSer-269phosphorylation.BiochemicalandBiophysicalResearchCommunications. 2017Jan22;482(4):524-529.PMID: 27889609
Gao,Y.,Stuart,D.,Pollock,J.S.,Takahashi,T.andKohan,D.E.,2016.Collectingduct-specificknockoutofnitricoxidesynthase3impairswaterexcretioninasex-dependentmanner.AmericanJournalofPhysiology-RenalPhysiology,pp.ajprenal-00494.PMID:27707708
Speed,J.S.,&Hyndman,K.A.(2016).Invivoorganspecificdrugdeliverywithimplantableperistalticpumps.Scientificreports,6.PMID:27185292
Choi,H.J.,Jung,H.J.,&Kwon,T.H.(2015).ExtracellularpHaffectsphosphorylationandintracellulartraffickingofAQP2ininnermedullarycollectingductcells.AmericanJournalofPhysiology-RenalPhysiology,308(7),F737-F748.PMID:25651562
MamenkoM,DhandeI,TomilinV,ZaikaO,BoukelmouneN,ZhuY,Gonzales-GarayML,PochynyukO,&Doris,P.(2015)DefectiveStore-OperatedCalciumEntryCausesPartialNephrogenicDiabetesInsipidus.J.Am.Soc.Nephrol.Nov16.PMID:26574044
Rice,W.L.,Li,W.,Mamuya,F.,McKee,M.,Păunescu,T.G.,&Lu,H.A.J.(2015).PolarizedTraffickingofAQP2RevealedinThreeDimensionalEpithelialCulture.PloSone,10(7),e0131719.PMID:26147297
Hoffert,J.D.,Pisitkun,T.,Saeed,F.,Wilson,J.L.,&Knepper,M.A.(2014).GlobalanalysisoftheeffectsoftheV2receptorantagoNISTsatavaptanonproteinphosphorylationincollectingduct.AmericanJournalofPhysiology-RenalPhysiology,306(4),410-421.PMID:24259510
Bogum,J.,Faust,D.,Zühlke,K.,Eichhorst,J.,Moutty,M.C.,Furkert,J.,EldahshanA,NeuenschwanderM,vonKriesJP,WiesnerB,&Trimpert,C.(2013).Small-moleculescreeningidentifiesmodulatorsofaquaporin-2trafficking.JournaloftheAmericanSocietyofNephrology,24(5),744-758.PMID: 23559583
HoffertJD,FentonRA,MoellerHB,SimonsB,TchapyjnikovD,McDillBW,YuMJ,PisitkunT,ChenF,KnepperMA.(2008)Vasopressin-stimulatedincreaseinphosphorylationatserine269potentiatesplasmamembraneretentionofaquaporin2.JBiolChem.2008Sep5;283(36):24617-27.PMID:18606813
