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PhosphoSolutions/ATF2 (Ser490,498) Antibody/p115-4908/100 µl
Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Human
- Applications:
- WB1:1000IHC1:1000
- Species:
- Rabbit
- MolecularReference:
- ~74kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p115-4908,RRID:AB_2492044
Antigen/Purification:Collapse
Theantigenisasyntheticphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser490,498ofhumanATF2.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
TheactivatingtranscriptionfactorATF2(alsocalledCRE-BP1)bindstobothAP-1andCREDNAresponseelementsandisamemberoftheATF/CREBfamilyofleucinezipperproteins(Maekawaetal.,1989).ATF2hasbeenimplicatedinthetranscriptionalregulationofanumberofgenesincludingcytokines,cellcyclecontrolandapoptosis.Variousformsofcellularstress,includinginflammatorycytokinesandUVirrADIation,stimulatethetranscriptionalactivityofATF2(Ivanovetal.,2003;Mortonetal.,2004).StressinducedATFdependenttranscriptionisdependentonphosphorylationofATF(Fuchsetal.,2000);Mortonetal.,2004).Serine490andserine498arenovelphosphorylationsitesonATFthathaverecentlybeenidentified.ATF2isparticularlyabundantinthebrainandtheATF2familyoftranscriptionfactorsisconsideredanimportantsubstrateofsignalsupstreamoftheactivationofgenesassociatedwithneuronalgrowthanddifferentiation(KarinandHunter,1995).ATFexpressionhasalsobeenlinkedtothedepressioninhumans(Laifenfeldetal.,2004).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Muñoz,DeniseP.,MisakoKawahara,andStevenM.Yannone.“Anautonomouschromatin/DNA-PKmechanismforlocalizedDNAdamagesignalinginmammaliancells.”Nucleicacidsresearch41.5(2013):2894-2906.PMID:23325849
BhoumikA,TakahashiS,BreitweiserW,ShilohY,JonesN,RonaiZ(2005)ATM-dependentphosphorylationofATF2isrequiredforDNAdamageresponse.MolCellMay27;18(5):577-87.PMID:15916964
