Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Zebrafish
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~34kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p135-15,RRID:AB_2492052
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Tyr15ofratcdc2.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Cdc2isahighlyconservedproteinserinekinasethatplaysakeyroleinregulationofthecellcycle(Maller,1991).TheABIlityofcdc2toexercisecontroloverthecellcycleisdependentuponthephosphorylationofTyr15incdc2(Nakamizoetal.,2002).cdc2expressioninbrainhasbeenlinkedtobothneurogenesisandapoptosis(KonishiandBonni,2003;Dranovskyetal.,2001;Okanoetal.,1996).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.StorageandStability.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
DranovskyA,VincentI,GregoriL,SchwarzmanA,ColfleshD,EnghildJ,StrittmatterW,DaviesP,GoldgaberD(2001)cdc2phosphorylationofnucleolindemarcatesmitoticstagesandAlzheimer’sdiseasepathology.NeurobiolAging22:517-528.
KonishiY,BonniA(2003)TheE2F-cdc2cell-cyclepathwayspecificallymediatesactivitydeprivation-inducedapoptosisofpostmitoticneurons.JNeurosci23:1649-1658.
MallerJL(1991)Mitoticcontrol.CurrOpinCellBiol3:269-275.
NakamizoA,InamuraT,InohaS,AmanoT,OchiH,IkezakiK,FukuiM(2002)Suppressionofcdc2dephosphorylationatthetyrosine15residueduringnitrosourea-inducedG2Mphasearrestinglioblastomacelllines.JNeurooncol59:7-13.
OkanoHJ,PfaffDW,GibbsRB(1996)ExpressionofEGFR-,p75NGFR-,andPSTAIR(cdc2)-likeimmunoreactivitybyproliferatingcellsintheadultrathippocampalformationandforebrain.DevNeurosci18:199-209.
