Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~110kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p111-94,RRID:AB_2492040
Antigen/Purification:Collapse
Theantigenisasyntheticphospho-peptidesurroundingthephosphoSer94ofhumanAR.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns
BIOLOGicalSignificance:Collapse
Theandrogenreceptor(AR)isaDNA-bindingtranscriptionfactorthatregulatesgenescriticalforthedevelopmentandmaintenanceofthemalesexualphenotype.Defectsinandrogenreceptorhavebeenshowntoplayaroleinprostatecancer,andinhibitionofARactivitythroughmodulationofsignaltransductionpathwaysmaydelayprostatecancerprogression(HeinleinandChang2004).Multiplephosphorylationsiteshavebeenidentifiedontheandrogenreceptorthataffectcross-talkbetweengrowthfactorsignalingandandrogeninprostatedevelopmentandcancer(Gioelietal.,2002).Oneofthesesites,atSer94,appearsconstitutivelyphosphorylatedandexhibitsnoresponsetotreatmentswithstimulatinghormone(Gioelietal.,2002).ThesiteatSer94isuniqueamongtheARphosphorylationsitesinthatitdoesnotachieveamaximallevelofphosphorylationbetweentranslationandtheinitialroundofnuclearimport,havingastrongbiasforandrogen-independentphosphorylationinthecytoplasm(Kesleretal.,2007).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
HeinleinC,ChangC.(2004)AndrogenReceptorinProstateCancer.EndocrineReviews.25(2):276-308
GioeliD,FicarroS,KwiekJ,AaronsonD,HancockM,CatlingA,WhiteF,ChristianR,SettlageR,ShabanowitzJ,HuntD,WeberM.(2002)AndrogenReceptorPhosphorylation,RegulationandIndentificationofthePhosphorylationSites.JBiolChem.277(32):29304-29314
KeslerC,GioeliD,ConawayM,WeberM,PaschalB.(2007)SubcellularLocalizationModulatesActivationFunction1DomainPhosphorylationintheAndrogenReceptor.MolecularEndocrinology.21(9):2071-2084
