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Functional_antibody >
PhosphoSolutions/Progesterone Receptor (Ser190) Antibody/p205-190/100 µl
Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Human
- Applications:
- WB1:1,000IHC1:1,000
- Species:
- Mouse
- MolecularReference:
- ~90kDaand~120kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p205-190,RRID:AB_2492215
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser190ofhumanprogesteronereceptor.
TheantibodyispreparedbyaffinitypurificationusingaProteinGcolumn.
BIOLOGicalSignificance:Collapse
Thereisaccumulatingevidencetosuggestthatprogesteroneplaysanessentialroleintheregulationofgrowthanddifferentiationofmammaryglandsandthusmayplayakeyroleinbreastcancer(Edwards,2005).Thebiologicalresponsetoprogesteroneismediatedbytwodistinctformsofthehumanprogesteronereceptor(PR-AandPR-Bforms).Inmostcellcontexts,theBformfunctionsasatranscriptionalactivator,whereastheAformfunctionsasatranscriptionalinhibitorofsteroidhormones(Attiaetal.,2000;Linetal.,2003).RecentlyithasbeendemonstratedthatthereisdifferentialhormonedependentregulationofthephosphorylationoftheAandBformsofthereceptor(Clemmetal.,2000).TreatmentofT47DbreastcancercellswithprogestinagoNISTincreasesthephosphorylationofSer190andSer294withdifferentkinetics.Thesephosphorylationeventsmaydifferentiallyaffectthetranscriptionalactivityofthereceptor.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Product SpecificReferences
López-Álvarez,V.M.,Modol,L.,Navarro,X.,Cobianchi,S.,&Neurodegenerativas,E.(2015).Earlyincreasing-intensitytreadmillexercisereducesneuropathicpainbypreventingnociceptorcollateralsproutinganddisruptionofchloridecotransportershomeostasisafterperipheralnerveinjury.Pain.Jun18.PMID:26090759
SilayevaL,DeebTZ,HinesM,KelleyMR,MunozMB,LeeHHC,BrandonNJ,DunlopJ,MaguireJ,DaviesPA,MossSJ.(2015)KCC2activityiscriticalinlimitingtheonsetandseverityofstatusepilepticus.PNAS.Mar2.pii:201415126.PMID:25733865
Mòdol,L.,Casas,C.,Llidó,A.,Navarro,X.,Pallarès,M.,&Darbra,S.(2014).NeonatalallopregnanoloneorfinasterideadministrationmodifieshippocampalK+Cl−co-transporterexpressionduringearlydevelopmentinmalerats.TheJournalofsteroidbiochemistryandmolecularbiology,143,343-347.PMID:24861264
Modol,L.,Cobianchi,S.,&Navarro,X.(2014).PreventionofNKCC1phosphorylationavoidsdownregulationofKCC2incentralsensorypathwaysandreducesneuropathicpainafterperipheralnerveinjury.PAIN,155(8),1577-1590.PMID:24813295
Mòdol,L.,Mancuso,R.,Alé,A.,Francos-Quijorna,I.,&Navarro,X.(2014).DifferentialeffectsonKCC2expressionandspasticityofALSandtraumaticinjuriestomotoneurons.Frontiersincellularneuroscience,8:7.PMID:24478630
SarkarJ,WakefieldS,MacKenzieG,MossSJandMaguireJ.(2011)Neurosteroidogenesisisrequiredforthephysiologicalresponsetostress:roleofneurosteroid-sensitiveGABAAreceptors.JNeurosci.31(50):18198-210.PMID:22171026
LeeHH,DeebTZ,WalkerJA,DaviesPA,MossSJ(2011)NMDAreceptoractivitydownregulatesKCC2resultingindepolarizingGABAAreceptor-mediatedcurrents.NatNeurosci.Jun;14(6):736-43.PMID:21532577
