Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:1,000IHC1:1,000
- Species:
- Mouse
- MolecularReference:
- ~90kDaand~120kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p205-294,RRID:AB_2492216
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer294ofhumanprogesteronereceptor.
TheantibodyispreparedbyaffinitypurificationusingaProteinGcolumn.
BIOLOGicalSignificance:Collapse
Thereisaccumulatingevidencetosuggestthatprogesteroneplaysanessentialroleintheregulationofgrowthanddifferentiationofmammaryglandsandthusmayplayakeyroleinbreastcancer(Edwards,2005).Thebiologicalresponsetoprogesteroneismediatedbytwodistinctformsofthehumanprogesteronereceptor(PR-AandPR-Bforms).Inmostcellcontexts,theBformfunctionsasatranscriptionalactivator,whereastheAformfunctionsasatranscriptionalinhibitorofsteroidhormones(Attiaetal.,2000;Linetal.,2003).RecentlyithasbeendemonstratedthatthereisdifferentialhormonedependentregulationofthephosphorylationoftheAandBformsofthereceptor(Clemmetal.,2000).TreatmentofT47DbreastcancercellswithprogestinagoNISTincreasesthephosphorylationofSer190andSer294withdifferentkinetics.Thesephosphorylationeventsmaydifferentiallyaffectthetranscriptionalactivityofthereceptor.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
AttiaGR,ZeitounK,EdwardsD,JohnsA,CarrBR,BulunSE(2000)ProgesteronereceptorisoformAbutnotBisexpressedinendometriosis.JClinEndocrinolMetab85:2897-2902.ClemmDL,ShermanL,BoonyaratanakornkitV,SchraderWT,WeigelNL,EdwardsDP(2000)Differentialhormone-dependentphosphorylationofprogesteronereceptorAandBformsrevealedbyaphosphoserinesite-specificmonoclonalantibody.MolEndocrinol14:52-65.
EdwardsDP(2005)Regulationofsignaltransductionpathwaysbyestrogenandprogesterone.AnnuRevPhysiol67:335-376.LinVC,WoonCT,AwSE,GuoC(2003)Distinctmolecularpathwaysmediateprogesterone-inducedgrowthinhibitionandfocaladhesion.Endocrinology144:5650-5657
