Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Bovine,Canine,GuineaPig
- Applications:
- WB1:1,000
- Species:
- Mouse
- MolecularReference:
- ~48kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1330-RXRA,RRID:AB_2492228
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheN-terminalregionofhumanretinoicacidreceptor,α-isotype.
TheantibodyispreparedfrommouseascitesbyammoniumsulfateprecipitationfollowedaffinitypurificationonaProteinGcolumn.
BIOLOGicalSignificance:Collapse
Retinoicacid(RA;activemetaboliteofvitaminA)playsaprominentroleinregulatingthetransitionofproliferatingprecursorcells(suchascarcinomacellsandneuronalprecursors)topostmitoticdifferentiatedcells(Joshietal.,2005).TheretinoidXreceptors(RXRs)family(RXRα,βandγ),preferentiallybind9-cis-RAandregulategenetranscriptionbyformingheterodimerswithasecondfamilyofRAreceptors.RAshavebeensuggestedtopotentiallyplayatherapeuticroleincervicalcancer(Abuetal.,2005).RAsareknowntoplaykeyrolesinneuronaldevelopmentandanincreasingbodyofevidenceindicatesthatretinoidsignalingmayregulatesynapticplasticityandassociatedlearningandmemorybehaviors(LaneandBailey,2005).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
AbuJ,BatuwangalaM,HerbertK,SymondsP(2005)Retinoicacidandretinoidreceptors:potentialchemopreventiveandtherapeuticroleincervicalcancer.LancetOncol6:712-720.
JoshiS,GuleriaR,PanJ,DipetteD,SinghUS(2005)Retinoicacidreceptorsandtissue-trans-glutaminasemediateshort-termeffectofretinoicacidonmigrationandinvasionofneuroblastomaSH-SY5Ycells.Oncogeneadvanceonlinepublication12September2005;doi:10.1038/sj.onc.1209027.
LaneMA,BaileySJ(2005)Roleofretinoidsignallingintheadultbrain.ProgNeurobiol75:275-293.
