Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Non-humanprimate,Canine,GuineaPig
- Applications:
- WB1:1,000
- Species:
- Mouse
- MolecularReference:
- ~48kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1340-RXRB,RRID:AB_2492229
Antigen/Purification:Collapse
Theantigenisa peptidecorrespondingtoaminoacidsfromN-terminalregionofhumanretinoicacidreceptor,β-isotype.Theantigenisidenticalinbothβ1andβ2isoforms.
TheantibodyispreparedfrommouseascitesbyammoniumsulfateprecipitationfollowedbyaffinitypurificationonaProteinGcolumn.
BIOLOGicalSignificance:Collapse
RetinoicAcid(RA;activemetaboliteofvitaminA)playsaprominentroleinregulatingthetransitionofproliferatingprecursorcells(suchascarcinomacellsandneuronalprecursors)topostmitoticdifferentiatedcells(Joshietal.,2005).TheRetinoidXreceptors(RXRs)family(RXRα,βandγ)preferentiallybind9-cis-RAandregulategenetranscriptionbyformingheterodimerswithasecondfamilyofRAreceptors(RARs).RAshavebeensuggestedtopotentiallyplayatherapeuticroleincervicalcancer(Abuetal.,2005).RAsareknowntoplaykeyrolesinneuronaldevelopmentandanincreasingbodyofevidenceindicatesthatretinoidsignalingmayregulatesynapticplasticityandassociatedlearningandmemorybehaviors(LaneandBailey,2005).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
AbuJ,BatuwangalaM,HerbertK,SymondsP(2005)Retinoicacidandretinoidreceptors:potentialchemopreventiveandtherapeuticroleincervicalcancer.LancetOncol6:712-720.
JoshiS,GuleriaR,PanJ,DipetteD,SinghUS(2005)Retinoicacidreceptorsandtissue-trans-glutaminasemediateshort-termeffectofretinoicacidonmigrationandinvasionofneuroblastomaSH-SY5Ycells.Oncogeneadvanceonlinepublication12September2005;doi:10.1038/sj.onc.1209027.
LaneMA,BaileySJ(2005)Roleofretinoidsignallingintheadultbrain.ProgNeurobiol75:275-293.
