Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000ICC1:50
- Species:
- Rabbit
- MolecularReference:
- ~35kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#317-CLAV,RRID:AB_2492058
Antigen/Purification:Collapse
Theantigenisa syntheticpeptidetakenfromtheC-terminusofratclavesin1.
Theantibodyispreparedfromserumbyaffinitypurificationusingacolumntowhichthepeptideantigenwascoupled.
BIOLOGicalSignificance:Collapse
Clavesin(clathrinvesicleassociatedSec14protein)isanovelneuronspecificproteinthathasrecentlybeenidentifiedandshowntoberequiredfornormalmorphologyoflateendosomesand/orlysosomesaslentiviral-mediatedknockdownofclavesininhippocampalneuronscauseslysosomaldefects(Katohetal.,2009).Additionally,upregulationofclavesininhumanhepatocellularcarcinomahasrecentlybeendemonstratedthusmakingitausefulMarkerforthisdiseasestate(Zhaoetal.,2008).
Storage
100µlinPBS+50%glycerol.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1yearafterdateofreceipt.Avoidfreeze/thawcycles.
GeneralReferences
YoheiKatoh,BrigitteRitter,ThomasGaffry,FrancoisBlondeau,StefanHöningandPeterS.McPherson(2009)theclavesinfamily:neuron-specificlipid-andclathrin-bindingSec14proteinsregulatinglysosomalmorphology.JournalofBiologicalChemistryOct2;284(40):27646-54.
ZhaoS,XuC,QianH,LvL,JiC,ChenC,ZhaoX,ZhengD,GuS,XieY,MaoY(2008)Cellularretinaldehyde-bindingprotein-like(CRALBPL),anovelhumanSec14p-likegenethatisupregulatedinhumanhepatocellularcarcinomas,maybeusedasamarkerforhumanhepatocellularcarcinomas.DNACellBiol.Mar;27(3):159-63.
