Sheeppolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000IF1:1,000IHC1:1,000
- Species:
- Sheep
- MolecularReference:
- ~95kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1050-774,RRID:AB_2492082
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser774ofratdynamin.
Theantibodyispreparedfrompooledsheepserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Dynaminisamemberofagroupofnerveterminalproteinscalleddephosphinsthatregulatesynapticvesicleendocytosis(Cousinetal.,2001;Grahametal.,2002;Tsuboietal.,2002).Cyclindependentproteinkinase5phosphorylatesdynaminatSer774andSer778 thatarethephosphorylationsitesondynaminphosphorylatedinvivo(Tanetal.,2003).PhosphorylationofSer774byGSK3hasrecentlybeenshowntocontrolactivity-dependentbulkendocytosisofsynapticvesicles(Claytonetal.,2010).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Vargas,K.J.,Schrod,N.,Davis,T.,Fernandez-BusnADIego,R.,Taguchi,Y.V.,Laugks,U.,Lucic,V.andChandra,S.S.,2017.SynucleinsHaveMultipleEffectsonPresynapticArchitecture.CellReports,18(1),pp.161-173. PMID:28052246
SergioLeal-Ortiz,ClarissaL.Waites,RyanTerry-Lorenzo,PedroZamorano,EckartD.Gundelfinger,andCraigC.Garner(2008)PiccolomodulationofSynapsin1adynamicsregulatessynapticvesicleexocytosis.J.CellBiol.,181:831–846.PMID:18519737
TanTC,ValovaVA,MalladiCS,GrahamME,BervenLA,JuppOJ,HansraG,McClureSJ,SarcevicB,BoadleRA,LarsenMR,CousinMA,RobinsonPJ(2003)cdk5isessentialforsynapticvesicleendocytosis.NatCellBiol5:701-710. PMID:12855954
