Sheeppolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Canine
- Applications:
- WB1:1,000
- Species:
- Sheep
- MolecularReference:
- ~95kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1050-778,RRID:AB_2492083
Antigen/Purification:Collapse
Theantigenisa phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser778ofratdynamin.
Theantibodyispreparedfrompooledsheepserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Dynaminisamemberofagroupofnerveterminalproteinscalleddephosphinsthatregulatesynapticvesicleendocytosis(Cousinetal.,2001;Grahametal.,2002;Tsuboietal.,2002).Cyclindependentproteinkinase5phosphorylatesdynaminatSer774andSer778thatarethephosphorylationsitesondynaminphosphorylatedinvivo(Tanetal.,2003).Phosphorylationofthesesitesondynaministhoughttoplayakeyroleinsynapticvesicletrafficking.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
SergioLeal-Ortiz,ClarissaL.Waites,RyanTerry-Lorenzo,PedroZamorano,EckartD.Gundelfinger,andCraigC.Garner(2008)PiccolomodulationofSynapsin1adynamicsregulatessynapticvesicleexocytosis.J.CellBiol.,181:831–846.PMID:18519737
