Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000IHC1:400
- Species:
- Rabbit
- MolecularReference:
- ~65kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1305-515,RRID:AB_2492156
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser513ofMunc-18.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Munc-18(mammalianhomologueofUnc-18)isaproteinthatisthoughttobeinvolvedinregulatingexocytosisdue,atleastinpart,toitABIlitytobindsyntaxin(Ciufoetal.,2005).Munc18-1isaneuron-specificmemberoftheSec1/Munc18proteinfamilythatbindstosyntaxin1Aandisthoughttostabilizethecomplex(Liuetal.,2004).ThefunctionofUnc-18isthoughttoberegulatedbyPKCphosphorylationofSer515inC.Elegans(Sassaetal.,1996).Ser513isthemammalianhomologueofSer515 inC.Elegans.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
SergioLeal-Ortiz,ClarissaL.Waites,RyanTerry-Lorenzo,PedroZamorano,EckartD.Gundelfinger,andCraigC.Garner(2008)PiccolomodulationofSynapsin1adynamicsregulatessynapticvesicleexocytosis.J.CellBiol.,181:831–846. PMID:18519737
