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Functional_antibody >
PhosphoSolutions/Munc18-1 (Ser241) Antibody/p1305-241/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Sheep,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~65kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1305-241,RRID:AB_2492155
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser241ofmouseMunc18-1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Munc-18(mammalianhomologueofUnc-18)isaproteinthatisthoughttobeinvolvedinregulatingexocytosisdue,atleastinpart,toitABIlitytobindsyntaxin(Ciufoetal.,2005).Munc18-1isaneuron-specificmemberoftheSec1/Munc18proteinfamilythatbindstosyntaxin1Aandisthoughttostabilizethecomplex(Liuetal.,2004).ThefunctionofMunc18-1isthoughttoberegulatedbyphosphorylation.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Wang,W.,Jia,Y.,Pham,D.T.,Palmer,L.C.,Jung,K.M.,Cox,C.D.,Rumbaugh,G.,Piomelli,D.,Gall,C.M.andLynch,G.,2017.AtypicalEndocannabinoidSignalingInitiatesaNewFormofMemory-RelatedPlasticityataCorticalInputtoHippocampus.CerebralCortex,pp.1-14.PMID:28520937
Meijer,M.,Cijsouw,T.,Toonen,R.F.,&Verhage,M.(2015).SynapticEffectsofMunc18-1AlternativeSplicinginExcitatoryHippocampalNeurons.PloSone,10(9),e0138950.PMID:26407320
