Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~82kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1825-RBP,RRID:AB_2492221
Antigen/Purification:Collapse
TheantigenisasyntheticpeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofrabphilin3A.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationonacolumntowhichtheC-terminalpeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Rabphilin-3AisaperipheralmembraneproteinthatbindsCa2+andphospholipidsandisattachedtosynapticvesiclemembranes(Geppertetal.,1994;Lietal.,1994).Theexpressionofrabphilin3AisreducedinananimalmodelofHuntington’sdisease(Smithetal.,2005).RabphilincanbephosphorylatedatSer234bybothCAMkinaseIIandPKAandthisphosphorylationhasbeensuggestedtoregulateneuronalactivityduringdevelopmentinasynapse-specificmanner(Fykseetal.,1995;FolettiandScheller,2001).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
FolettiDL,SchellerRH(2001)Developmentalregulationandspecificbraindistributionofphosphorabphilin.JNeurosci21:5461-5472.
FykseEM,LiC,SudhofTC(1995)Phosphorylationofrabphilin-3AbyCa2+/calmodulin-andcAMPdependentproteinkinasesinvitro.JNeurosci15:2385-2395.
GeppertM,BolshakovVY,SiegelbaumSA,TakeiK,DeCamilliP,HammerRE,SüdhofTC(1994)TheroleofRab3Ainneurotransmitterrelease.Nature(Lond)369:493-497.
LiC,TakeiK,GeppertM,DaniellL,SteniusK,ChapmanER,JahnR,DeCamilliP,SüdhofTC(1994)Synaptictargetingofrabphilin-3A,asynapticvesicleCa2+/phospholipid-bindingprotein,dependsonrab3A/3C.Neuron13:885-898.
SmithR,PetersenA,BatesGP,BrundinP,LiJY(2005)Depletionofrabphilin3Ainatransgenicmousemodel(R6/1)ofHuntington’sdisease,apossIBLeculpritinsynapticdysfunction.NeurobiolDis20:673-684.
