Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Mostmammals
- Applications:
- WB1:1,000IHC1:100
- Species:
- Mouse
- MolecularReference:
- ~39kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1840-RHO,RRID:AB_2492232
Antigen/Purification:Collapse
Theantigenisabovinerhodopsin.
TheantibodyisProteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
Rhodopsinisaphotoreceptorproteinfoundinretinalrods.Itisacomplexformedbythebindingofretinaldehyde,theoxidizedformofretinol,totheproteinopsinandundergoesaseriesofcomplexreactionsinresponsetovisIBLelightresultinginthetransmissionofnerveimpulsestothebrain.Mutationoftherhodopsingeneisamajorcontributortovariousretinopathiessuchasretinitispigmentosa.Thedisease-causingproteingenerallyaggregateswithubiquitinininclusionbodies,disruptstheintermediatefilamentnetworkandimpairstheABIlityofthecelltodegradenon-functioningproteinswhichleadstophotoreceptorapoptosis(Bersonetal.,1991).OthermutationsonrhodopsinleadtoX-linkedcongenitalstationarynightblindness,mainlyduetoconstitutiveactivation,whenthemutationsoccuraroundthechromophorebindingpocketofrhodopsin(Dryjaetal.,1993).Severalotherpathologicalstatesrelatingtorhodopsinhavebeendiscoveredincludingpoorpost-Golgitrafficking,dysregulativeactivation,rodoutersegmentinstabilityandarrestinbinding.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
ProductSpecificReferences
