Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:500
- Species:
- Rabbit
- MolecularReference:
- ~34kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1610-2325,RRID:AB_2492227
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr23/25ofhumanREDD1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
REDD1,RegulatedinDevelopmentandDNAdamageresponses1,isinducedbyhypoxia,cellstress,andapoptosis.ReducedREDD1levelscansensitizecellstowardsapoptosis,whereelevatedlevelsofREDD1inducedbyhypoxiacandesensitizecellstoapoptoticstimuli(Schwarzeretal,2005).REDD1hasacrucialroleininhibitingmammalianrapamycincomplex1(mTORC1)signalingduringhypoxicstress(Katiyaretal,2009).IthasbeenshownthattherapiddegradationofREDD1ismediatedbytheCUL4A–DDB1–ROC1–b-TRCPE3ligasecomplexandisregulatedbyREDD1phosphorylationatThr25,Thr23andSer19throughtheactivityofGSK3b(Katiyaretal,2009).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
Schwarzerr,TonderaD,ArnoldW,GieseK,KlippelA,KaufmannJ(2005)REDD1integrateshypoxia-mediatedsurvivalsignalingdownstreamofphosphatidylinositol3-kinase.Oncogene24(7):1138-49.
KatiyarS,LiuE,KnutzenCA,LangES,LombardoCR,SankarS,TothJI,PetroskiMD,RonaiZ,ChiangGC(2009)REDD1,aninhibitorofmTORsignaling,isregulatedbytheCUL4A-DDB1ubiquitinligase.EMBORep10(8):866-72.
