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Functional_antibody >
PhosphoSolutions/Troponin I (cardiac) (Ser23/24) Antibody/p2010-2324/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate
- Applications:
- WB1:2,000
- Species:
- Rabbit
- MolecularReference:
- ~25kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p2010-2324,RRID:AB_2492268
Antigen/Purification:Collapse
Phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser23/24ofmousetroponinI,cardiac(cTnI).
Preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphosphoandde-phosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
TroponinI(cTnI)is1of3subunits,alongwithtroponinC(TnC)andtroponinT(TnT)oftroponincomplexfoundincardiacmuscle.cTnIbindstoactininthinmyofilamentstoholdthetroponin-tropomyosincomplexinplace.PhosphorylationofcardiacisoformofTnIatserines22,23intheuniqueamino-terminalendmoleculedecreasesthecalciumsensitivityofthesarcomere,promotescalciumdissociationfromtroponinCandbyextensionenhancesratesofcross-bridgecyclinganddiastolicrelaxation(Noland,Jr.etal.,1995;Nolandetal.,1989).Inaddition,studiesusingreconstitutedfibersandmutationalanalysishaveshownthatPKCphosphorylationofTnI(largelyatSer43)inhibitstheactin-crossbridgereactionandreducestheCa++dependentactomyosinATPaserateaswellasthecalciumsensitivityofforcegeneration(Noland,Jr.andKuo,1991).PhosphorylationatThr144(mediatedbyseveralPKCisoforms)reducesmaximaltensiondevelopmentandcross-bridgecyclingrates(Sumandeaetal.,2008).Importantly,changesinthephosphorylationateachofthesesiteshavebeenshowntobestage-specificwithregardtocardiacdiseaseprogression(Walkeretal.,2010).
Storage
100μlneatserum.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Thoemmes,S.F.,Stutzke,C.A.,Du,Y.,Browning,M.D.,Buttrick,P.M.,&Walker,L.A.(2014).Characterizationandvalidationofnewtoolsformeasuringsite-specificcardiactroponinIphosphorylation.Journalofimmunologicalmethods,403(1),66-71.PMID:24291343
