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PhosphoSolutions/Troponin I (cardiac) Antibody/2010-TNI/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- Serapooled,neatserum
- Specificity:
- Rat,Mouse
- Applications:
- WB1:2,000
- Species:
- Rabbit
- MolecularReference:
- ~25kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2010-TNI,RRID:AB_2492267
Antigen/Purification:Collapse
Theantigenisa fusionproteinofthemousecardiactroponinIholoprotein.
Theantibodyisunpurifiedneatserum.
BIOLOGicalSignificance:Collapse
TroponinI(cTnI)is1of3subunits,alongwithtroponinC(TnC)andtroponinT(TnT)oftroponincomplexfoundincardiacmuscle.cTnIbindstoactininthinmyofilamentstoholdthetroponin-tropomyosincomplexinplace.PhosphorylationofcardiacisoformofTnIatserines22,23intheuniqueamino-terminalendmoleculedecreasesthecalciumsensitivityofthesarcomere,promotescalciumdissociationfromtroponinCandbyextensionenhancesratesofcross-bridgecyclinganddiastolicrelaxation(Noland,Jr.etal.,1995;Nolandetal.,1989).Inaddition,studiesusingreconstitutedfibersandmutationalanalysishaveshownthatPKCphosphorylationofTnI(largelyatSer43)inhibitstheactin-crossbridgereactionandreducestheCa++dependentactomyosinATPaserateaswellasthecalciumsensitivityofforcegeneration(Noland,Jr.andKuo,1991).PhosphorylationatThr144(mediatedbyseveralPKCisoforms)reducesmaximaltensiondevelopmentandcross-bridgecyclingrates(Sumandeaetal.,2008).Importantly,changesinthephosphorylationateachofthesesiteshavebeenshowntobestage-specificwithregardtocardiacdiseaseprogression(Walkeretal.,2010).
Storage
100μlneatserum.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
NolandTA,Jr.,GuoXD,RaynorRL,JideamaNM,Averyhart-FullardV,SolaroRJ,KuoJF(1995)CardiactroponinImutants–PhosphorylationbyproteinkinasesCandAandregulationofCa2+-stimulatedMgATPaseofreconstitutedactomyosinS-1.JBiolChem270:25445-25454.
NolandTA,Jr.,KuoJF(1991)ProteinkinaseCphosphorylationofcardiactroponinIortroponinTinhibitsCa2(+)-stimulatedactomyosinMgATPaseactivity.JBiolChem266:4974-4978.
NolandTAJr,RaynorRL,KuoJF(1989)IdentificationofsitesphosphorylatedinbovinecardiactroponinIandtroponinTbyproteinkinaseCandcomparativesubstrateactivityofsyntheticpeptidescontainingthephosphorylationsites.JBiolChem264:20778-20785.
SumandeaMP,RybinVO,HinkenAC,WangC,KobayashiT,HarletonE,SievertG,BalkeCW,FeinmarkSJ,SolaroRJ,SteinbergSF(2008)TyrosinephosphorylationmodifiesproteinkinaseCdelta-dependentphosphorylationofcardiactroponinI.JBiolChem283:22680-22689.
WalkerLA,WalkerJS,AmblerSK,ButtrickPM(2010)Stage-specificchangesinmyofilamentproteinphosphorylationfollowingmyocardialinfarctioninmice.JMolCellCardiol48:1180-1186
