Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~25kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p2010-150,RRID:AB_2492270
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer150ofmousetroponinI,cardiac(cTnI).
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
TroponinI(TnI)is1of3subunits,alongwithtroponinC(TnC)andTroponinT(TnT)oftroponincomplexfoundincardiac(cTnI)andfastskeletal(fsTnI)muscle.cTnIisphosphorylatedbyproteinkinaseCandproteinkinaseAatSer23/24(Nolandetal,1995)andisphosphorylatedbyAMPKatSer23andSer150(Solisetal,2011).EvidencesuggeststhatAMPK,acriticalregulatorofcardiacenergetics,prefersphosphorylatingSer150overSer23,andmayplayaroleinregulatingenergyconsumptionthroughalteringthephosphorylationstatusofcTnI(Solisetal.,2011).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Thoemmes,S.F.,Stutzke,C.A.,Du,Y.,Browning,M.D.,Buttrick,P.M.,&Walker,L.A.(2014).Characterizationandvalidationofnewtoolsformeasuringsite-specificcardiactroponinIphosphorylation.Journalofimmunologicalmethods,403(1),66-71.PMID:24291343
