Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~250kDaand~150kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p2400-295,RRID:AB_2492287
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser295ofmouseXIRP1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
XIRP1isamemberoftheXinfamilyofproteins,containing16Xinrepeats.TheintercalateddiskproteinXinprotectsactinfilamentsfromdepolymerization,andisabletobundleactinfilamentstointeractwithβ-catenin,whichisthoughttostABIlizeactin-basedCytoskeletons(Choietal.,2007).TheevolutionaryemergenceoftheXinparalogsmayhaveplayedakeyroleinthedevelopmentofheartchamberswithcompleteendothelialandmyocardiallayers(Grosskurthetal.,2008).LossofmXina(mouseXinhomolog)resultsincardiachypertrophyandcardiomyopathy(Gustafson-Wagneretal.,2007).PhosphorylationofSer295isplaysakeyroleinXIRP1signaling,andthereforeincardiachypertrophy,asithasbeenshownthatacutepressure-overloadedheartsrapidlyincreasedphosphorylationattheSer295residueofXIRP1.(Changetal.,2013).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20° Cforatleast1year.
ProductSpecificReference
Chang,Y.W.,Chang,Y.T.,Wang,Q.,Lin,J.J.C.,Chen,Y.J.,&Chen,C.C.(2013).Quantitativephosphoproteomicstudyofpressure-overloadedmouseheartrevealsdynamin-relatedprotein1asamodulatorofcardiachypertrophy.Molecular&CellularProteomics,12(11),3094-3107. PMID:23882026
