Sheeppolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mouse,Human,Non-humanprimate
- Applications:
- WB1:1,000
- Species:
- Sheep
- GeneName:
- DBH
- MolecularReference:
- ~75kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#420-DBHC,RRID:AB_2492074
Antigen/Purification:Collapse
TheantigenisapeptidefromtheC-terminalregionofhumandopamineβ-hydroxylase(DBH),conjugatedtokeyholelimpethemocyanin(KLH).
TheantibodyispreparedfrompooledsheepserumbyaffinitypurificationusingaSulfo-Link®columnmatrixtowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
DBHcatalyzestheconversionofdopaminetonorepinephrineandservesasaMarkerofnoradrenergiccells.DBHantibodiesandantibodiesforothermarkersofcatecholaminebiosynthesisarewidelyusedasmarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).TheexpressionofDBHisalsoelevatedduringstress(SabbanandKvetnansky,2001).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
ZhuMY,KlimekV,HaycockJW,OrdwayGA(2000)Quantitationoftyrosinehydroxylaseproteininthelocuscoeruleusfrompostmortemhumanbrain.JNeurosciMeth99:37-44.PMID:10936640
