Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Non-humanprimate
- Applications:
- WB1:1,000IF1:1,000IHC1:1,000
- Species:
- Rabbit
- GeneName:
- TH
- MolecularReference:
- ~60kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1580-31,RRID:AB_2492278
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingphospho-Ser31ofrattyrosinehydroxylase(TH).
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Tyrosinehydroxylase(TH)istherate-limitingenzymeinthesynthesisofthecatecholaminesdopamineandnorepinephrine.THantibodiescanthereforebeusedasMarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).THantibodiescanalsobeusedtoexplorebasicmechanismsofdopamineandnorepinephrinesignaling(Witkovskyetal.,2000;Salvatoreetal.,2001;Dunkleyetal.,2004).TheactivityofTHisalsoregulatedbyphosphorylation(Haycocketal.,1982;Haycocketal.,1992;Jedynaketal.,2002).Phospho-specificantibodiesforthephosphorylationsitesonTHcanbeusedtogreateffectinstudyingthisregulationandinidentifyingthecellsinwhichTHphosphorylationoccurs.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Jorge-Finnigan,A.,Kleppe,R.,Jung-KC,K.,Ying,M.,Marie,M.,Rios-Mondragon,I.,Salvatore,M.F.,Saraste,J.andMartinez,A.,2017.Phosphorylationatserine31targetstyrosinehydroxylasetovesiclesfortransportalongmicrotubules.JournalofBiologicalChemistry,pp.jbc-M116.PMID:28637871
Bonito-Oliva,Alessandra,GiadaSpigolon,andGilbertoFisone.(2015)“RegulationofPre-andPostsynapticProteinPhosphorylationbyDopamineD2Receptors.”DopamineReceptorTechnologies.SpringerNewYork,2015.79-100.
Izumi,Y.,Ezumi,M.,Takada-Takatori,Y.,Akaike,A.,&Kume,T.(2014).Endogenousdopamineisinvolvedintheherbicideparaquat-induceddopaminergiccelldeath.ToxicologicalSciences,139(2),466-478.PMID:24743698
Calipari,E.S.,Ferris,M.J.,Melchior,J.R.,Bermejo,K.,Salahpour,A.,Roberts,D.,&Jones,S.R.(2014).Methylphenidateandcocaineself‐admiNISTrationproducedistinctdopamineterminalalterations.Addictionbiology,19(2),145-155.PMID:22458761
JedynakJP,AliSF,HaycockJW,HopeBT(2002)Acuteadministrationofcocaineregulatesthephosphorylationofserine-19,-31and-40intyrosinehydroxylase.JNeuRochem82:382-388.PMID:12124439
SalvatoreMF,WaymireJC,HaycockJW(2001)Depolarization-stimulatedcatecholaminebiosynthesis:involvementofproteinkinasesandtyrosinehydroxylasephosphorylationsitesinsitu.JNeurochem79:349-360.PMID:11677263
WitkovskyP,GabrielR,HaycockJW,MellerE(2000)Influenceoflightandneuralcircuitryontyrosinehydroxylasephosphorylationintheratretina.JChemNeuroanat19:105-116.PMID:10936746
