Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mostmammals
- Applications:
- WB1:1,000IF1:1000IHC1:1000
- Species:
- Rabbit
- GeneName:
- TH
- MolecularReference:
- ~60kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1580-40,RRID:AB_2492279
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser40ofrattyrosinehydroxylase.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Tyrosinehydroxylase(TH)istherate-limitingenzymeinthesynthesisofthecatecholaminesDopamineandNorepinephrine.THantibodiescanthereforebeusedasMarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).THantibodiescanalsobeusedtoexplorebasicmechanismsofdopamineandnorepinephrinesignaling(Witkovskyetal.,2000;Salvatoreetal.,2001;Dunkleyetal.,2004).TheactivityofTHisalsoregulatedbyphosphorylation(Haycocketal.,1982;Haycocketal.,1992;Jedynaketal.,2002).Phospho-specificantibodiesforthephosphorylationsitesonTHcanbeusedtogreateffectinstudyingthisregulationandinidentifyingthecellsinwhichTHphosphorylationoccurs.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Salvatore,M.F.,Terrebonne,J.,Cantu,M.A.,McInnis,T.R.,Venable,K.,Kelley,P.,Kasanga,E.A.,Latimer,B.,Owens,C.L.,Pruett,B.S.andYu,Y.,2017.Dissociationofstriataldopamineandtyrosinehydroxylaseexpressionfromaging-relatedmotordecline:evidencefromcalorierestrictionintervention.JournalsofGerontologySeriesA:BiomedicalSciencesandMedicalSciences,p.glx119.PMID:28637176
Jorge-Finnigan,A.,Kleppe,R.,Jung-KC,K.,Ying,M.,Marie,M.,Rios-Mondragon,I.,Salvatore,M.F.,Saraste,J.andMartinez,A.,2017.Phosphorylationatserine31targetstyrosinehydroxylasetovesiclesfortransportalongmicrotubules.JournalofBiologicalChemistry,pp.jbc-M116.PMID:28637871
Barroso-Chinea,P.,Cruz-Muros,I.,Afonso-Oramas,D.,Castro-Hernández,J.,Salas-Hernández,J.,Chtarto,A.,Luis-Ravelo,D.,Humbert-Claude,M.,Tenenbaum,L.andGonzález-Hernández,T.(2016)Long-termcontrolledGDNFover-expressionreducesdopaminetransporteractivitywithoutaffectingtyrosinehydroxylaseexpressionintheratmesostriatalsystem.NeurobiologyofDisease.88:44-54. PMID:26777664
Bonito-Oliva,A.,Spigolon,G.,&Fisone,G.(2015).RegulationofPre-andPostsynapticProteinPhosphorylationbyDopamineD2Receptors.InDopamineReceptorTechnologies(pp.79-100).SpringerNewYork.
Calipari,E.S.,Ferris,M.J.,Melchior,J.R.,Bermejo,K.,Salahpour,A.,Roberts,D.,&Jones,S.R.(2014).Methylphenidateandcocaineself‐admiNISTrationproducedistinctdopamineterminalalterations.Addictionbiology,19(2),145-155.PMID:22458761
Izumi,Y.,Ezumi,M.,Takada-Takatori,Y.,Akaike,A.,&Kume,T.(2014).Endogenousdopamineisinvolvedintheherbicideparaquat-induceddopaminergiccelldeath.ToxicologicalSciences,139(2),466-478.PMID:24743698
Salvatore,M.F.(2014).ser31tyrosinehydroxylasephosphorylationparallelsdifferencesindopaminerecoveryinnigrostriatalpathwayfollowing6‐OHDAlesion.JournalofneuRochemistry,129(3),548-558.PMID:24410633
McCutcheonJE,ConradKL,CarrSB,FordKA,McGeheeDS,andMarinelliM(2012)Dopamineneuronsintheventraltegmentalareafirefasterinadolescentratsthaninadults.JNeurophysiology108(6):1620-1630.PMID:22723669
MeyerDA,RicherE,BenkovicSA,HayashiK,KansyJW,HaleCF,MoyLY,KimY,O’CallaghanJP,TsaiLH,GreengardP,NairnAC,CowanCW,MillerDB,AntichP,andBibbJA(2008)StriataldysregulationofCdk5alterslocomotorresponsestococaine,motorlearning,anddendriticmorphology.PNAS,105:18561–18566.PMID:19017804
JedynakJP,AliSF,HaycockJW,HopeBT(2002)Acuteadministrationofcocaineregulatesthephosphorylationofserine-19,-31and-40intyrosinehydroxylase.JNeurochem82:382-388.PMID:12124439
SalvatoreMF,WaymireJC,HaycockJW(2001)Depolarization-stimulatedcatecholaminebiosynthesis:involvementofproteinkinasesandtyrosinehydroxylasephosphorylationsitesinsitu.JNeurochem79:349-360.PMID:11677263
WitkovskyP,GabrielR,HaycockJW,MellerE(2000)Influenceoflightandneuralcircuitryontyrosinehydroxylasephosphorylationintheratretina.JChemNeuroanat19:105-116.PMID:10936746
