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PhosphoSolutions/Tyrosine Hydroxylase Antibody, Rabbit/2025-THRAB/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mostmammals,Someinvertebrates
- Applications:
- WB1:1,000IF1:1000IHC1:1000
- Species:
- Rabbit
- GeneName:
- TH
- MolecularReference:
- ~60kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2025-THRAB,RRID:AB_2492276
Antigen/Purification:Collapse
Theantigenisan SDS-denaturedrattyrosinehydroxylase,purifiedfrompheochromocytoma.
Preparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Tyrosinehydroxylase(TH)istherate-limitingenzymeinthesynthesisofthecatecholaminesdopamineandnorepinephrine.THantibodiescanthereforebeusedasMarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).THantibodiescanalsobeusedtoexplorebasicmechanismsofdopamineandnorepinephrinesignaling(Witkovskyetal.,2000;Salvatoreetal.,2001;Dunkleyetal.,2004).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
deRusJacquet,A.,Timmers,M.,Ma,S.Y.,Thieme,A.,McCabe,G.P.,Vest,J.H.C.,Lila,M.A.andRochet,J.C.,2017.LumbeetrADItionalmedicine:NeuroprotectiveactivitiesofmedicinalplantsusedtotreatParkinson’sdisease-relatedsymptoms.JournalofEthnopharmacology.Feb15.PMID:28214539
deRusJacquet,A.,Tambe,M.A.,Ma,S.Y.,McCabe,G.P.,Vest,J.H.C.andRochet,J.C.,2017.Pikuni-Blackfeettraditionalmedicine:NeuroprotectiveactivitiesofmedicinalplantsusedtotreatParkinson’sdisease-relatedsymptoms.JournalofEthnopharmacology. Jan11.PMID:28088492
KishSJ,KalasinskyKS,DerkachP,SchmunkGA,GuttmanM,AngL,AdamsV,FurukawaY,HaycockJW(2001)StriataldopaminergicandSEROtonergicmarkersinhumanheroinusers.Neuropsychopharmacology24:561-567.PMID:11282256
SalvatoreMF,WaymireJC,HaycockJW(2001)Depolarization-stimulatedcatecholaminebiosynthesis:involvementofproteinkinasesandtyrosinehydroxylasephosphorylationsitesinsitu.JNeurochem79:349-360.PMID:11677263
WitkovskyP,GabrielR,HaycockJW,MellerE(2000)Influenceoflightandneuralcircuitryontyrosinehydroxylasephosphorylationintheratretina.JChemNeuroanat19:105-116. PMID:10936746
ZhuMY,KlimekV,HaycockJW,OrdwayGA(2000)Quantitationoftyrosinehydroxylaseproteininthelocuscoeruleusfrompostmortemhumanbrain.JNeurosciMeth99:37-44. PMID:10936640
ZhuMY,KlimekV,DilleyGE,HaycockJW,StockmeierC,OverholserJC,MeltzerHY,OrdwayGA(1999)Elevatedlevelsoftyrosinehydroxylaseinthelocuscoeruleusinmajordepression.BiolPsychiatry46:1275-1286.PMID:10560033
XuZQ,LewJY,HaradaK,AmanK,GoldsteinM,DeutchA,HaycockJW,HokfeltT(1998)Immunohistochemicalstudiesonphosphorylationoftyrosinehydroxylaseincentralcatecholamineneuronsusingsite-andphosphorylationstate-specificantibodies.Neurosci82:727-738.PMID:9483531
