Sheeppolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mostmammals,Someinvertebrates
- Applications:
- WB1:1,000IF1:1,000IHC1:1,000
- Species:
- Sheep
- GeneName:
- TH
- MolecularReference:
- ~60kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2027-THSHP,RRID:AB_2492277
Antigen/Purification:Collapse
Theantigenisa nativerattyrosinehydroxylase,purifiedfrompheochromocytoma.
Theantibodyispreparedfromsheepserumbyaffinitypurificationusingacolumntowhichimmunogenwascoupled.TheantibodyispredominantlyoftheIgG1subclass.
BIOLOGicalSignificance:Collapse
Tyrosinehydroxylase(TH)istherate-limitingenzymeinthesynthesisofthecatecholaminesdopamineandnorepinephrine.THantibodiescanthereforebeusedasMarkersfordopaminergicandnoradrenergicneuronsinavarietyofapplicationsincludingdepression,schizophrenia,Parkinson’sdiseaseanddrugabuse(Kishetal.,2001;Zhuetal.,2000;Zhuetal.,1999).THantibodiescanalsobeusedtoexplorebasicmechanismsofdopamineandnorepinephrinesignaling(Witkovskyetal.,2000;Salvatoreetal.,2001).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
ZhuMY,KlimekV,HaycockJW,OrdwayGA(2000)Quantitationoftyrosinehydroxylaseproteininthelocuscoeruleusfrompostmortemhumanbrain.JNeurosciMeth99:37-44.PMID:10936640
