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PhosphoSolutions/Vesicular Monoamine Transporter 2 Antibody, C-terminus/2250-VMAT2C/100 µl
Sheeppolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Human
- Applications:
- WB1:1,000
- Species:
- Sheep
- MolecularReference:
- ~57kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2250-VMAT2C,RRID:AB_2492286
Antigen/Purification:Collapse
TheantigenisapeptidefromintracellularC-terminalregionofhumanVMAT2,conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyispreparedfromsheepserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Vesicularneurotransmittertransporterssequesterthetransmittersintosynapticvesicles(Ericksonetal.,1996).Thevesicularmonoaminetransporter2(VMAT2)isresponsIBLeforcatecholamineandSEROtoninstorageincentralsynapses.AntibodiesspecificforVMAThavebeenusedtomonitorexpressionofthetransporterduringdevelopmentandinagingandcanbeeffectivelyusedasaMarkerformonoamineterminals(Haycocketal.,2003;Witkovskyetal.,2005).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
ProductSpecificReferences
Ivanova,E.,Yee,C.W.,&Sagdullaev,B.T.(2015).Disruptionindopaminergicinnervationduringphotoreceptordegeneration.JournalofComparativeNeurology.Sep10.PMID:26356010
