Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~17kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1008-58,RRID:AB_2492065
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser58ofmouseCOXIVprotein.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
COX,alsoknownascytochromecoxidase,has12subunitsthatmakeupthetransmembranemitochondrialprotein.SubunitIVhastwoisoforms;COXIV-1andCOXIV-2(Huttermannetal.,2001).COXIV-1isexpressedubiquitouslywhileCOXIV-2ishighlyexpressedinadultlungandlowlevelsinbrainandheart(Huttermannetal.,2001).PhosphorylationofaminoacidresidueSer58oftheCOXIV-1proteinisaPKA-dependentregulationofCOXandplaysanimportantroleinmetabolismandCREBcycleactivation(AcinPerezetal.,2011).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Acin-PerezR,GattiD,BaiY,ManfrediD(2011)ProteinPhosphorylationandPreventionofCytochromeOxidaseInhibitionbyATP:CoupledMechanismsofEnergyMetabolismRegulation.CellMetabolism13,712-719.PMID:21641552
