Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Bovine
- Applications:
- WB1:5,000IF1:1,000
- Species:
- Mouse
- MolecularReference:
- ~35kDaand~45kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#451-DCX,RRID:AB_2492079
Antigen/Purification:Collapse
TheantigenisafulllengthrecombinanthumandoublecortinexpressedinandpurifiedfromE.coli.
TheantibodyisProteinGpurifiedfromculturesupernatant.
BIOLOGicalSignificance:Collapse
Doublecortin,orDCX,isamicrotubuleassociatedproteinthatisexpressedalmostexclusivelyinveryearlyneuronaldevelopment(Brownetal.,2003),makingitanexcellentMarkerfordevelopingneuronalcells.DefectsintheDCXgeneleadtoX-linkedlissencephalywhichischaracterizedbyalackofnormalfoldsonthesurfaceofthebrainresultinginasmoothcerebralcortexcausedbyabnormalmigrationofneuronsduringdevelopment(desPortesetal.,1998;Gleesonetal.,1998).
Storage
100µlinPBS+10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
BrownJP,Couillard-DesprésS,Cooper-KuhnCM,WinklerJ,AignerL,KuhnHG(2003)Transientexpressionofdoublecortinduringadultneurogenesis.JCompNeurol.Dec1;467(1):1-10.
desPortesV,PinardJM,BilluartP,VinetMC,KoulakoffA,CarriéA,GelotA,DupuisE,MotteJ,Berwald-NetterY,CatalaM,KahnA,BeldjordCandChellyJ.(1998)AnovelCNSgenerequiredforneuronalmigrationandinvolvedinX-linkedsubcorticallaminarheterotopiaandlissencephalysyndrome.Cell92:51-61.
GleesonJG,AllenKM,FoxJW,LampertiED,BerkovicS,SchefferI,CooperEC,DobynsWB,MinnerathSR,RossMEandWalshCA.(1998)Doublecortin,abrain-specificgenemutatedinhumanX-linkedlissencephalyanddoublecortexsyndrome,encodesaputativesignalingprotein.Cell92:63-72.
