Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgGfraction
- Specificity:
- Human,Mouse,Rat,MostMammals,Drosophila
- Applications:
- WB1:1,000IF1:500
- Species:
- Mouse
- MolecularReference:
- ~34kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#560-FIB,RRID:AB_2492093
Antigen/Purification:Collapse
Theantigenisayeastnuclearpreparations.
TheantibodyistotalIgGfraction.
BIOLOGicalSignificance:Collapse
Nop1pwasoriginallyidentifiedasanucleolarproteinofbakersyeast,Saccharomycescerevisiae.TheNop1pproteinis327aminoacidsinsize(34.5kDa),isessentialforyeastviABIlity,andislocalizedinthenucleoli(1).ThesystematicnameforS.cerevisiaeNop1isYDL014W,anditisnowknowntobepartofthesmallsubunitprocessomecomplex,involvedintheprocessingofpre-18SribosomalRNA.Nop1pistheyeasthomologueofaproteinfoundinalleukaryotesandarchaeagenerallycalledfibrillarin(2).Fibrillarin/Nop1pisextraordinarilyconserved,sothattheyeastandhumanproteinsare67%identical,andthehumanproteincanfunctionallyreplacetheyeastprotein.Patientswiththeautoimmunediseasesclerodermaoftenhavestrongcirculatingautoantibodiestoa~34kDaproteinwhichwassubsequentlyfoundtobefibrillarin.Recentstudiesshowthatknock-outofthefibrillaringeneinmiceresultsinembryoniclethality,althoughmicewithonlyonefunctionalfibrillarin/Nop1pgenewereviable(3).ThisantibodyisbecomingwidelyusedasaconvenientMarkerfornucleoliinawidevarietyofspecies(e.g.4-6).
Storage
Liquid.Contains10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
OchsRL,LischweMA,SpohnWH,BuschH.Fibrillarin:anewproteinofthenucleolusidentifiedbyautoimmunesera.BiolCell54:123-133(1985).
ArisJPandBlobelG.Identificationandcharacterizationofayeastnucleolarproteinthatissimilartoaratlivernucleolarprotein.J.CellBiol.107:17-31(1988).
NewtonK,PetfalskiE,TollerveyD,CaceresJF.Fibrillarinisessentialforearlydevelopmentandrequiredforaccumulationofanintron-encodedsmallnucleolarRNAinthemouse.MolCellBiol.23:8519-8527(2003).
TyagiSandAlsmADIO.Imagingnativebeta-actinmRNAinmotilefibroblasts.BiophysJ.87:4153-62(2004).
Paeschke1K,SimonssonT,PostbergJ,RhodesD,LippsH-J.Telomereend-bindingproteinscontroltheformationofG-quadruplexDNAstructuresinvivoNatureStructural&MolecularBiology12,847-854(2005).
VermaakD,HenikoffS,MalikHS.Positiveselectiondrivestheevolutionofrhino,amemberoftheheterochromatinprotein1familyinDrosophila.PLoSGenetics1:96-108(2005).
