Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgMfraction
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Porcine
- Applications:
- WB1:1,000IF1:100
- Species:
- Mouse
- MolecularReference:
- ~38kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#600-GAPDH,RRID:AB_2492132
Antigen/Purification:Collapse
TheantigenisanextensivelypurifiedpigGAPDH.
Theantibodyisunpurified,concentratedculturesupernatant.
BIOLOGicalSignificance:Collapse
Glyceraldehyde3-PhosphateDehydrogenase(GAPDH)isametabolicenzymeresponsIBLeforcatalyzingonestepintheglycolyticpathway,thereversibleoxidativephosphorylationofglyceraldehyde3-phosphate.BecauseGAPDHisaproteinexpressedinlargeamountsandwhichisrequiredatalltimesforimportant“housekeeping”functions,levelsofGAPDHmRNAareoftenmeasuredandusedasstandardsinstudiesofmRNAexpression.Increasingly,scientistsaremakinguseofspecificantibodiestoGAPDHincomparablestudiesoflevelsofproteinexpression.ThisantibodycanbeusedasaloADIngcontrolforwesternblottingexperiments,allowingcomparisonbetweenthelevelofthisproteinandothersinacellortissue.Apartfromaroleinglycolysis,GAPDHmayhaveotherrolessuchasintheactivationoftranscription(1).GAPDHisreportedtobindtoavarietyofotherproteins,includingtheamyloidprecursorprotein,mutationsinwhichcausesomeformsofAlzheimer’sdisease,andthepolyglutaminetractsofHuntingtin,theproteinproductaberrantformsofwhicharecausativeofHuntington’sdisease(2,3).Associationswithactinandtubulinhavealsobeenreported.Theproteinmayalsohavearoleintheregulationofapoptosis,andinterestinglymigratesfromthecytoplasmintothenucleuswhencellsbecomeapoptotic(4).
Storage
Liquid.Contains10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
MorgeneggG,WinklerGC,HubscherU,HeizmannCW,MousJ,KuenzleCC.Glyceraldehyde-3-phosphatedehydrogenaseisanonhistoneproteinandapossibleactivatoroftranscriptioninneurons.JNeuRochem.47:54-62(1986).
SchulzeH,SchulerA,StuberD,DobeliH,LangernH&HuberG.Ratbrainglyceraldehyde-3-phosphatedehydrogenaseinteractswiththerecombinantcytoplasmicdomainofAlzheimer’sbeta-amyloidprecursorprotein.JNeurochem.60:1915-22(1993).
BurkeJR,EnghildJJ,MartinME,JouY-S,MyersRM,RosesAD,VanceJM&StrittmatterWJ.HuntingtinandDRPLAproteinsselectivelyinteractwiththeenzymeGAPDH.NatureMed.2:347-350(1996).
DastoorZ.&Dreyer,J-L.Potentialroleofnucleartranslocationofglyceraldehyde-3-phosphatedehydrogenaseinapoptosisandoxidativestress.J.CellSci.114:1643-1653(2001).
