Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Neatserum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:5,000IF1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~50kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#620-GFAP,RRID:AB_2492124
Antigen/Purification:Collapse
TheantigenisarecombinantandpurifiedbovineGFAP.
Theantibodyisunpurifiedneatserum.
BIOLOGicalSignificance:Collapse
GlialFibrillaryAcidicProtein(GFAP)wasdiscoveredbyAmicoBignamiandco-workersasamajorfibrousproteinofmultiplesclerosisplaques(1).Itwassubsequentlyfoundtobeamemberofthe10nmorintermediatefilament(IF)family,specificallytheIFfamilyClassIII,whichalsoincludesperipherin,desminandvimentin.GFAPisstronglyandspecificallyexpressedinastrocytesandcertainotherastrogliaintheCNS,insatellitecells,peripheralganglia,andinnon-myelinatingSchwanncellsinperipheralnerves.InmanydamageanddiseasestatesGFAPexpressionisheavilyupregulatedinastrocytes.Inaddition,neuralstemcellsfrequentlystronglyexpressGFAP.PointmutationsintheproteincodingregionoftheGFAPgeneleadtoAlexanderdiseasewhichischaracterizedbythepresenceofabnormalastrocytescontainingGFAPproteinaggregatesknownasRosenthalfibers(2).
Storage
100µlneatserum.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
BignamiA,EngLF,DahlD,UyedaCT.Localizationoftheglialfibrillaryacidicproteininastrocytesbyimmunofluorescence.BrainRes.43:429-35(1972).
BrennerM,JohnsonAB,Boespflug-TanguyO,RodriguezD,GoldmanJEandMessingA.MutationsinGFAP,encodingglialfibrillaryacidicprotein,areassociatedwithAlexanderdisease.NatGenet27:117-20(2001).
