Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Zebrafish
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~87kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1185-1526,RRID:AB_2492143
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser152,156ofMARCKS.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
MyristoylatedAlanine-RichCKinaseSubstrate(MARCKS)isamajorsubstrateforphosphorylationbyproteinkinaseC(PKC)(Ouimetetal.,1990).ThephosphorylationofSer152/156canbeusedasameasureofPKCactivationalthoughthesesitesarealsophosphorylatedbyPRK1(Palmeretal.,1996)MARCKSisamemberofafamilyofcalmodulinbindingproteinsandphosphorylationofSer152/156modulatesthebindingofMARCKStocalmodulin(Vergheseetal.,1994)
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
OuimetCC,WangJKT,WalaasSI,AlbertKA,GreengardP(1990)LocalizationoftheMARCKS(87kDa)protein,amajorspecificsubstrateforproteinkinaseC,inratbrain.JNeurosci10:1683-1698.
PalmerRH,SchönwasserDC,RahmanD,PappinDJC,HergetT,ParkerPJ(1996)PRK1phosphorylatesMARCKSatthePKCsites:Serine152,serine156andserine163.FEBSLett378:281-285.
VergheseGM,JohnsonJD,VasulkaC,HauptDM,StumpoDJ,BlackshearPJ(1994)ProteinkinaseC-mediatedphosphorylationandcalmodulinbindingofrecombinantmyristoylatedalanine-richCkinasesubstrate(MARCKS)andMARCKS-relatedprotein.JBiolChem269:9361-9367.
