Chickenpolyclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgYfraction
- Specificity:
- Rat,Mouse,Human,Bovine
- Applications:
- WB1:20,000IF1:2,500
- Species:
- Chicken
- MolecularReference:
- ~280kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1100-MAP2,RRID:AB_2492141
Antigen/Purification:Collapse
TheantigenisarecombinantbovineMAP2expressedinandpurifiedfromE.coli.
TheantibodyistotalIgYfraction.
BIOLOGicalSignificance:Collapse
Microtubulesare25nmdiameterproteinrodsfoundinmostkindsofeukaryoticcells.Theyarepolymerizedfromadimericsubunitmadeofoneasubunitandonebtubulinsubunit.Microtubulesareassociatedwithafamilyofproteinscalledmicrotubuleassociatedproteins(MAPs),whichincludestheproteinτ (tau)andagroupofproteinsreferredtoasMAP1,MAP2,MAP3,MAP4andMAP5(Kindler&Gardner1994).MAP2ismadeupoftwo~280kDaapparentmolecularweightbandsreferredtoasMAP2aandMAP2b.Athirdlowermolecularweightform,usuallycalledMAP2c,correspondstoapairofproteinbandsrunningat~70kDaonSDS-PAGEgels.AlltheseMAP2formsarederivedfromasinglegenebyalternatetranscription,andallshareaC-terminalsequencewhichincludeseitherthreeorfourmicrotubulebindingpeptidesequences,whichareverysimilartothosefoundintherelatedmicrotubulebindingproteinτ (tau).MAP2isoformsareexpressedonlyinneuronalcellsandspecificallyintheperikaryaanddendritesofthesecells.MAP2hasbeenrecentlyshowntobethespecificreceptorfortheneurosteroidpregnenolone(Fontaine-LenoreV.etal.,2006).
Storage
Liquidcontaining10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
ProductSpecificReferences
