Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Bovine
- Applications:
- WB1:5,000IF1:2,500IHC1:2,500
- Species:
- Mouse
- MolecularReference:
- ~70kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1101-MAP2C,RRID:AB_2571638
Antigen/Purification:Collapse
TheantigenisafulllengthrecombinanthumanMAP2Dprotein.
Theantibodyis ProteinGpurified.
BIOLOGicalSignificance:Collapse
Microtubulesare25nmdiameterproteinrodsfoundinmostkindsofeukaryoticcells.Microtubulesareassociatedwithafamilyofproteinscalledmicrotubuleassociatedproteins(MAPs),whichincludestheprotein(tau)andagroupofproteinsreferredtoasMAP1,MAP2,MAP3,MAP4andMAP5(Kindler&Gardner1994).MAP2ismadeupoftwo~280kDabandsreferredtoasMAP2aandMAP2b.Athirdlowermolecularweightform,MAP2CandMAP2D,correspondstoapairofproteinbandsrunningat~70kDaonSDS-PAGEgels.AlltheseMAP2formsarederivedfromasinglegenebyalternatetranscription,andallshareaC-terminalsequencewhichincludeseitherthreeorfourmicrotubulebindingpeptidesequences,whichareverysimilartothosefoundintherelatedmicrotubulebindingprotein(tau).MAP2isoformsareexpressedonlyinneuronalcellsandspecificallyintheperikaryaanddendritesofthesecells.MAP2CandMAP2DareexpressedearlierindevelopmentthantheMAP2aandMAP2bisoforms,sothatthisantibodyisamoreusefulMarkerofneuronaldevelopment.MAP2hasbeenrecentlyshowntobethespecificreceptorfortheneurosteroidpregnenolone(FontaineLenoreV.etal.,2006).
Storage
100µlinPBS+50%glyceroland5mMsodiumazide.
Forlongtermstorage–20°Cisrecommended.Storeat–20°Cinundilutedaliquots;stableforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
KindlerS,GardnerCC(1994)FourrepeatMAP2isoformsinhumanandratbrain.BrainResMolBrainRes.26(1-2):218-224.
Fontaine-LenoreV,ChambraudB,FellousA,DavidS,DuchossoyY,BaulieuEE,RobelP(2006)Microtubule-associatedprotein2(MAP2)isaneurosteroid.ProcNatlAcadSciUSA103(12):4711-6.
