Chickenpolyclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgYfraction
- Specificity:
- Rat,Mouse,Human,Chicken
- Applications:
- WB1:50,000IF1:25,000
- Species:
- Chicken
- MolecularReference:
- ~200kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1451-NFH,RRID:AB_2492160
Antigen/Purification:Collapse
TheantigenisapurifiedbovineNF-H.
TheantibodyistotalIgYfraction.
BIOLOGicalSignificance:Collapse
Neurofilamentsarethe10nmorintermediatefilamentproteinsfoundspecificallyinneurons,andarecomposedpredominantlyofthreemajorproteinscalledNF-L,NF-MandNF-H(Harrisetal.,1991).NF-HistheneurofilamenthighorheavymolecularweightpolypeptideandrunsonSDS-PAGEgelsat200-220kDa,withsomevariABIlityacrossspeciesboundaries.AntibodiestoNF-Hareusefulforidentifyingneuronalcellsandtheirprocessesintissuesectionsandintissueculture.NF-Hantibodiescanalsobeusefultovisualizeneurofilamentaccumulationsseeninmanyneurologicaldiseases,suchasAmyotrophicLateralSclerosis(LouGehrig’sdisease)(Mendoncaetal.,2005)andAlzheimer’sdisease(Huetal.,2002).
Storage
Liquidcontaining10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
Harris,J.,Ayyub,C.andShawG.(1991)AmoleculardissectionofthecarboxyterminaltailsofthemajorneurofilamentsubunitsNF-MandNF-H.JNeurosciRes30:47-62.
MendoncaDM,ChimelliL,MartinezAM.(2005)Quantitativeevidenceforneurofilamentheavysubunitaggregationinmotorneuronsofspinalcordsofpatientswithamyotrophiclateralsclerosis.BrazJMedBiolRes.38(6):925-933.
HuYY,HeSS,WangXC,DuanQH,KhatoonS,IgbalK,Grundke-IgbalI,WangJZ(2002)ElevatedlevelsofphosphorylatedneurofilamentproteinsincerebrospinalfluidofAlzheimerdiseasepatients.NeurosciLett320(3):156-60.
