Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgGfraction
- Specificity:
- Chicken,Mostmammals
- Applications:
- WB1:5,000IF1:500
- Species:
- Mouse
- MolecularReference:
- ~200kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1450-NFH,RRID:AB_2492159
Antigen/Purification:Collapse
TheantigenisapurifiedbovineneurofilamentH.
Theantibodyisunpurifiedmouseascitesfluid.
BIOLOGicalSignificance:Collapse
Neurofilamentsarethe10nmorintermediatefilamentproteinsfoundspecificallyinneurons,andarecomposedpredominantlyofthreemajorproteinscalledNF-L,NF-MandNF-H(1).NF-HistheneurofilamenthighorheavymolecularweightpolypeptideandrunsonSDS-PAGEgelsat200-220kDa,withsomevariABIlityacrossspeciesboundaries.AntibodiestoNF-Hareusefulforidentifyingneuronalcellsandtheirprocessesintissuesectionsandintissueculture.NF-Hantibodiescanalsobeusefultovisualizeneurofilamentaccumulationsseeninmanyneurologicaldiseases,suchasAmyotrophicLateralSclerosis(LouGehrig’sdisease)(2)andAlzheimer’sdisease(3).
Storage
100µlmouseascitesfluid.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
Harris,J.,Ayyub,C.andShawG.(1991)AmoleculardissectionofthecarboxyterminaltailsofthemajorneurofilamentsubunitsNF-MandNF-H.JNeurosciRes30:47-62.
MendoncaDM,ChimelliL,MartinezAM.(2005)Quantitativeevidenceforneurofilamentheavysubunitaggregationinmotorneuronsofspinalcordsofpatientswithamyotrophiclateralsclerosis.BrazJMedBiolRes.38(6):925-933.
HuYY,HeSS,WangXC,DuanQH,KhatoonS,IgbalK,Grundke-IgbalI,WangJZ(2002)ElevatedlevelsofphosphorylatedneurofilamentproteinsincerebrospinalfluidofAlzheimerdiseasepatients.NeurosciLett320(3):156-60.
