Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgGfraction
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken
- Applications:
- WB1:1,000IF1:100
- Species:
- Mouse
- MolecularReference:
- ~68kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1452-NFL,RRID:AB_2492161
Antigen/Purification:Collapse
Theantigenisapreparationofporcineintermediatefilaments.
Theantibodyisunpurified,concentratedculturesupernatant.
BIOLOGicalSignificance:Collapse
Neurofilamentsarethe10nmorintermediatefilamentproteinsfoundspecificallyinneurons,andarecomposedpredominantlyofthreemajorproteinscalledNF-L,NF-MandNF-H(1).NF-ListheneurofilamentlightorlowmolecularweightpolypeptideandrunsonSDS-PAGEgelsatabout~68kDa.AntibodiestoNF-Lareusefulforidentifyingneuronalcellsandtheirprocessesintissuesectionsandintissueculture.MutationsintheproteincodingregionofthehumanNF-LgenecausesomeformsofCharcot-Marie-Toothdisease(2).
Storage
Liquidcontaining10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
Harris,J.,Ayyub,C.andShawG.(1991)AmoleculardissectionofthecarboxyterminaltailsofthemajorneurofilamentsubunitsNF-MandNF-H.JNeurosciRes30:47-62.
MersiyanovaIV,PerepelovAV,PolyakovAV,SitnikovVF,DadaliEL,OparinRB,PetrinANandEvgrafovOV.(2000)AnewvariantofCharcot-Marie-Toothdiseasetype2isprobablytheresultofamutationintheneurofilament-lightgene.Am.J.Hum.Genet.67:37-46.
