Chicken polyclonal antibody
- Size:
- 100 µl
- Formulation:
- Total IgY fraction
- Specificity:
- Rat, Mouse, Human, Chicken
- Applications:
- WB 1:5,000 IF 1:1,000
- Species:
- Chicken
- Molecular Reference:
- ~145 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 1455-NFM, RRID:AB_2492164
Antigen/Purification: Collapse
The antigen is a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
The antibody is total IgY fraction.
Biological Significance: Collapse
Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Harris et al., 1991). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig’s disease) and Alzheimer’s disease (Hu et al., 2002).
Storage
Liquid containing 10mM sodium azide.
For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.
General References
Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.
