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PhosphoSolutions/Neurofilament M Antibody, Mouse/1454-NFM/100 µl
Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgGfraction
- Specificity:
- Rat,Mouse,Human,Chicken
- Applications:
- WB1:1,000IF1:100
- Species:
- Mouse
- MolecularReference:
- ~145kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1454-NFM,RRID:AB_2492163
Antigen/Purification:Collapse
TheantigenisarecombinantfusionproteincontainingtheextremeC-terminusofratNF-MexpressedinandpurifiedfromE.coli.
Theantibodyisunpurified,concentratedculturesupernatant.
BIOLOGicalSignificance:Collapse
Neurofilamentsarethe10nmorintermediatefilamentproteinsfoundspecificallyinneurons,andarecomposedpredominantlyofthreemajorproteinscalledNF-L,NF-MandNF-H(1).NF-MistheneurofilamentmiddleormediummolecularweightpolypeptideandrunsonSDS-PAGEgelsat145-160kDa,withsomevariABIlityacrossspeciesboundaries.AntibodiestoNF-Mareusefulforidentifyingneuronalcellsandtheirprocessesintissuesectionsandintissueculture.NF-Mantibodiescanalsobeusefultovisualizeneurofilamentaccumulationsseeninmanyneurologicaldiseases,suchasAmyotrophicLateralSclerosis(LouGehrig’sdisease)andAlzheimer’sdisease(2).
Storage
Liquidcontaining10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
Harris,J.,Ayyub,C.andShawG.(1991)AmoleculardissectionofthecarboxyterminaltailsofthemajorneurofilamentsubunitsNF-MandNF-H.JNeurosciRes30:47-62.
HuYY,HeSS,WangXC,DuanQH,KhatoonS,IgbalK,Grundke-IgbalI,WangJZ(2002)ElevatedlevelsofphosphorylatedneurofilamentproteinsincerebrospinalfluidofAlzheimerdiseasepatients.NeurosciLett320(3):156-60.
