Chickenpolyclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgYfraction
- Specificity:
- Mostmammals
- Applications:
- WB1:1,000IF1:500
- Species:
- Chicken
- MolecularReference:
- ~57kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1631-PER,RRID:AB_2492208
Antigen/Purification:Collapse
TheantigenisarecombinantratperipherinexpressedinandpurifiedfromE.coli.
TheantibodyistotalIgYfraction.
BIOLOGicalSignificance:Collapse
Peripherinisa~57kDaintermediatefilamentsubunitfoundinitiallyinsensoryneuronsoftheperipheralnervoussystems,whichgivestheproteinitsname.Subsequently,peripherinwasfoundinsomesensoryandotherneuronsofthecentralnervoussystemandalsoinPC12cells.Peripherinisalsoexpressedincertainneuroendocrinetumorsandintheinsulinproducingcellsofthepancreas.PeripherinbelongstotheClassIIIfamilyofintermediatefilamentsubunitswhichalsoincludesvimentin,glialfibrillaryacidicprotein(GFAP)anddesmin.Incontrasttotheneurofilaments,peripherinisstronglyup-regulatedafternerveinjury(1).Antibodiestoperipherincanbeusedinidentifying,classifying,andstudyingneuronsthroughoutthenervoussystem.PeripherinisalsoagooddiagnosticMarkerforballoonedaxonsseeninLouGehrig’sdisease(Amyotrophiclateralsclerosis)andsomeneuronallyderivedtumors(2).Autoantibodiestoperipherinarefrequentlyseenintheseraofpatientswithdiabetes(3).Peripherinisnotrelatedtoperipherin/RDS,aproteinofthephotoreceptoroutermembranemutationsofwhicharecausativeofcertainformsofslowretinaldegeneration.
Storage
Liquid.Contains10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
TeraoE,JanssensS,vandenBoschdeAquilarP,PortierM,KlosenP(2000)Invivoexpressionoftheintermediatefilamentperipherininratmotorneurons:modulationbyinhibitoryandstimulatorysignals.Neuroscience101(3):679-88.
RobersonJ,DoroudchiMM,NguyenMD,Durham,HD,ShawG,JulienJP,MushynskiWE.(2003)AneurotoxicperipherinsplicevariantinamousemodelofALS.J.CellBiol.160(6):939-49.
BoitardC,VillaMC,BecourtC,GiaHP,HucC,SempeP,PortierMM,BachJF(1992)Peripherin:anisletantigenthatiscross-reactivewithnonobesediabeticmouseclassIIgeneproducts.ProcNatlAcadSciUSA89(1):172-6.
