Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Bovine
- Applications:
- WB1:2,000IF1:1,000
- Species:
- Mouse
- MolecularReference:
- ~49kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1900-SAP,RRID:AB_2492236
Antigen/Purification:Collapse
TheantigenisarecombinantfulllengthhumanSAP49expressedinandpurifiedfromE.Coli.
TheantibodyisProteinGpurifiedfromtissueculturesupernatant.
BIOLOGicalSignificance:Collapse
Thespliceosomeassociatedproteinof~49kDa(SAP49),alsoknownasSF3B4,isawidelyexpressednuclearsplicingfactorthathasbeenshowntohaveRNAbindingactivityaswellasdirectinteractionwithSAP145(Champion-ArnaudandReed1994).TheSAP145-SAP49complexhasrecentlybeenimplicatedincellcycleprogressionasVpr(viralprotein1ofHIV-1)hasbeenshowntobindSAP145thusinterferingwiththeproperformationandfunctioningoftheSAP145-SAP49complex(TeradaandYasuda2006).
Storage
100µlliquidcontaining10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
Champion-ArnaudP,ReedR.(1994)TheprespliceosomecomponentsSAP49andSAP145interactinacompleximplicatedintetheringU2snRNPtothebranchsite.GenesDev.8(16):1974-83.
TeradaY,YasudaY.(2006)Humanimmunodeficiencyvirustype1VprinducesG2checkpointactivationbyinteractingwiththesplicingfactorSAP145.MolCellBiol.26(21):8149-58
