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PhosphoSolutions/Ubiquitin C-terminal Hydrolase 1 (PGP9.5) Antibody, Chicken/2061-UCHL1/100 µl
Chickenpolyclonalantibody
- Size:
- 100µl
- Formulation:
- TotalIgYfraction
- Specificity:
- Rat,Mouse,Human,Bovine
- Applications:
- WB1:2,500IF1:500
- Species:
- Chicken
- MolecularReference:
- ~24kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2061-UCHL1,RRID:AB_2492281
Antigen/Purification:Collapse
Theantigenis a recombinantfulllengthhumanUCHL1purifiedfromE.coli.
Theantibodyis TotalIgYfraction.
BIOLOGicalSignificance:Collapse
UbiquitinC-terminalhydrolase1(UCHL1)isalsoknownasubiquitincarboxylesteraseL1,ubiquitinthiolesterase,neuron-specificproteinPGP9.5andPark5.Itwasoriginallyidentifiedasamajorcomponentoftheneuronalcytoplasmfrom2-dimensionalgelanalysisofbraintissues,andwasgiventhenamePGP9.5(1).ItwaslaterfoundthatubiquitinC-terminalhydrolaseenzymeactivitywasassociatedwiththePGP9.5protein(2).TheubiquitinC-terminalhydrolasescleaveubiquitinfromothermolecules.Regulationoftheubiquitinpathwayisveryimportantandmanydiseasestatesareassociatedwithdefectsinthispathway.GeneticknockoutofUCHL1inmiceresultsinamotorneurondegenerationsimilartothespontaneousgracileaxonaldystrophy(gad)mutantmice(3).PointmutationsintheUCHL1geneareassociatedwithsomeformsofhumanParkinson’sdisease(4).SinceUCHL1isheavilyexpressedinneurons,itisreleasedinlargeamountsfollowinginjuryordegeneration,sothedetectionofUCHL1inCSFandotherbodilyfluidscanbeusedasabioMarker.
Storage
Liquid.Contains10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
DoranJF,JacksonP,KynochPA,ThompsonRJ.IsolationofPGP9.5,anewhumanneurone-specificproteindetectedbyhigh-resolutiontwo-dimensionalelectrophoresis.JNeuRochem.40:1542-7(1983).
WilkinsonKD,LeeKM,DeshpandeS,Duerksen-HughesP,BossJM,PohlJ.TheneuronspecificproteinPGP9.5isaubiquitincarboxyl-terminalhydrolase.Science.1989246:670-3(1989).
KuriharaLJ,KikuchiT,WadaK,TilghmanSM.LossofUch-L1andUch-L3leadstoneurodegeneration,posteriorparalysisanddysphagia.HumMolGenet.10:1963-70(2001).
LiuY,FallonL,LashuelHA,LiuZ,LansburyPTJr.TheUCH-L1geneencodestwoopposingenzymaticactivitiesthataffectalpha-synucleindegradationandParkinson’sdiseasesusceptibility.Cell111:209-18(2002)
