Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Bovine
- Applications:
- WB1:1,000IF1:500
- Species:
- Mouse
- MolecularReference:
- ~50kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2107-VIM,RRID:AB_2492284
Antigen/Purification:Collapse
TheantigenisarecombinanthumanvimentinpurifiedfromE.coli.
TheantibodyisProteinGpurified.
BIOLOGicalSignificance:Collapse
Vimentinisthemajorproteinsubunitofthe10nmorintermediatefilaments(IFs)foundinmanykindsofmesenchymalandepithelialcellsaswellasdevelopingneuronalandastrocyticprecursorcellsintheCNS.Vimentinisthoughttobecriticallyinvolvedinlymphocyteadhesionandtransmigration(NieminenMetal.2006).CopolymersarefrequentlyformedbetweenvimentinandotherIFs,suchasGFAP(inmanykindsofastrocytes),desmin(inmusclecells)andneurofilamentproteins(indevelopingneurons).AntibodiestovimentinareusefulinstudiesofstemcellsandgenerallytorevealthefilamentousCytoskeleton.Recentstudiessuggestthatvimentinaffectsprostatecancercellsmotilityandinvasiveness(Zhaoetal.2008).
Storage
Liquid.Contains10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
References
NieminenM,HenttinenT,MerinenM,Marttila-IchiharaF,ErikssonJE,JalkanenS(2006)Vimentinfunctioninlymphocyteadhesionandtranscellularmigration.NatCellBiol8(2):156-62.
ZhaoY,YanQ,LongX,ChenX,WangY(2008)Vimentinaffectsthemobilityandinvasivenessofprostatecancercells.CellBiochemFunct.May8[Epubaheadofprint]
