Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Bovine
- Applications:
- WB1:2,000IF1:500
- Species:
- Mouse
- MolecularReference:
- ~22kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2145-VSNL1,RRID:AB_2492285
Antigen/Purification:Collapse
TheantigenisrecombinantfulllengthhumanVSNL1expressedinandpurifiedfromE.Coli.
TheantibodyisProteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
Visinin-likeprotein1(VSNL1),alsoknownasVILIP1,isacalciumsensorproteinexpressedexclusivelyinneurons.HighestlevelsofVSNL1expressionarefoundincerebellarPurkinjecells.VSNL1hasbeenimplicatedinthemodulationofcellsignalingcascadesviaregulationofadenylcyclaseactivity(Braunewelletal.,1997).Additionally,VSNL1hasbeenassociatedwithamyloidplaquesandneurofibrillartanglesinAlzheimer’sdisease(Schnurraetal.,2001).
Storage
100ulliquidcontaining10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
BraunewellKH,SpilkerC,BehnischT,GundelfingerED(1997)Theneuronalcalcium-sensorproteinVILIPmodulatescyclicAMPaccumulationinstablytransfectedC6gliomacells:amino-terminalmyristoylationdeterminesfunctionalactivity.J.NeuRochem.68(5):2129–39.
SchnurraI,BernsteinHG,RiedererP,BraunewellKH(2002)TheneuronalcalciumsensorproteinVILIP-1isassociatedwithamyloidplaquesandextracellulartanglesinAlzheimer’sdiseaseandpromotescelldeathandtauphosphorylationinvitro:alinkbetweencalciumsensorsandAlzheimer’sdisease?Neurobiol.Dis.8(5):900–9.
