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PhosphoSolutions/GABAA Receptor α6 Antibody, 50 µl/850-GA6/50 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 50µl
- Formulation:
- Serapooled,neatserum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GABRA6
- MolecularReference:
- ~57kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#850-GA6,RRID:AB_2492106
Antigen/Purification:Collapse
Theantigenisapeptiderepresentingasequencethatisspecificfortheα6-subunitofratGABAAreceptor.PeptideantigenhasnohomologywithanyotherGABAAR-subunit.
Theantibodyisunpurifiedneatserum.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAAreceptor(GABAA-R)subtype.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsubstanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzodiazepinemodulation.Thevariouseffectsofthebenzodiazepinesinbrainmayalsobemediatedviadifferentα-subunitsofthereceptor(McKernanetal.,2000;MehtaandTicku,1998;Ogrisetal.,2004;Pöltletal.,2003).Lastly,phosphorylationofβ-subunitsofthereceptorhasbeenshowntomodulateGABAA-Rfunction(Brandonetal.,2003).
Storage
50µlneatserum.Adequatematerialtoconduct10mini-Westernblots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
BrandonNJ,JovanovicJN,ColledgeM,KittlerJT,BrandonJM,ScottJD,MossSJ(2003)Akinaseanchoringprotein79/150facilitatesthephosphorylationofGABAAreceptorsbycAMP-dependentproteinkinaseviaselectiveinteractionwithreceptorβ-subunits.MolCellNeurosci22:87-97.
McKernanRM,etal.(2000)SedativebutnotanxiolyticpropertiesofbenzodiazepinesaremediatedbytheGABAAreceptorα1-subtype.NatureNeurosci3:587-592.
MehtaAK,TickuMK(1998)PrevalenceoftheGABAAreceptorassembliescontainingα1-subunitintheratcerebellumandcerebralcortexasdeterminedbyimmunoprecipitation:LackofmodulationbychronicethanoladmiNISTration.MolBrainRes67:194-199.
OgrisW,PöltlA,HauerB,ErnstM,ObertoA,WulffP,HögerH,WisdenW,SieghartW(2004)AffinityofvariousbenzodiazepinesiteligandsinmicewithapointmutationintheGABAAreceptorγ2-subunit.BiochemPharmacol68:1621-1629.OlsenRW,TobinAJ(1990)MolecularbiologyofGABAAreceptors.FASEB4:1469-1480.
PöltlA,HauerB,FuchsK,TretterV,SieghartW(2003)SubunitcompositionandquantitativeimportanceofGABAAreceptorsubtypesinthecerebellumofmouseandrat.JNeuRochem87:1444-1455.
WhitingPJ,BonnertTP,McKernanRM,FarrarS,LeBourdellèsB,HeavensRP,SmithDW,HewsonL,RigbyMR,SirinathsinghjiDJS,ThompsonSA,WaffordKA(1999)MolecularandfunctionaldiversityoftheexpandingGABAAreceptorgenefamily.AnnNYAcadSci868:645-653
