>
产品中心 >
Functional_antibody >
PhosphoSolutions/GABAA Receptor β3 (Ser408/409) Antibody/p1130-4089/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GABRB3
- MolecularReference:
- ~58kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1130-4089,RRID:AB_2492111
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthedi-phospho-Ser408/409oftheratGABAAreceptor,β3subunit.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem.TherearetwomajorclassesofGABAreceptors:theGABAA andtheGABAB subtypeofreceptors.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsubstanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzodiazepinemodulation.Phosphorylationofserine408andserine409withintheβ3subunithavebeenshowntobecriticalforthefunctionalmodulationofβ3containingrecombinantreceptors(Brandonetal.,2000).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Riffault,B.,Medina,I.,Dumon,C.,Thalman,C.,Ferrand,N.,Friedel,P.,Gaiarsa,J.L.,&Porcher,C.(2014).Pro-Brain-DerivedNeurotrophicFactorInhibitsGABAergicNeurotransmissionbyActivatingEndocytosisandRepressionofGABAAReceptors.TheJournalofNeuroscience,34(40),13516-13534.PMID:25274828
