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Functional_antibody >
PhosphoSolutions/GABAA Receptor γ2 (Ser327) Antibody/p1130-327/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GABRG2
- MolecularReference:
- ~45kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1130-327,RRID:AB_2492116
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser327oftheGABAAreceptor,γ2subunit.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem.TherearetwomajorclassesofGABAreceptors:theGABAAandtheGABAABsubtypeofreceptors.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsubstanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzodiazepinemodulation.IthasrecentlybeensuggestedthatPKCΕregulatesthesensitivityofGABAAα1β2γ2receptorstoethanolandbenzodiazepinesthroughphosphorylationofserine327inthelargeintracellularloopofγ2(Qietal.,2007)
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Bohnsack,J.P.,Carlson,S.L.,&Morrow,A.L.(2016).Differentialregulationofsynapticandextrasynapticα4GABA(A)receptorpopulationsbyproteinkinaseAandproteinkinaseCinculturedcorticalneurons.Neuropharmacology,105,124-132.PMID:26767953
QiZH,SongM,WallaceMJ,WangD,NewtonPM,McMahonT,ChouWH,ZhangC,ShokatKM,MessingRO(2007)ProteinkinaseCεregulatesγ-aminobutyratetypeAreceptorsensitivitytoethanolandbenzodiazepinesthroughphosphorylationofγ2subunits.JBiolChem282(45):33052-63.PMID:17875639
